Stimulustriggered fate conversion of somatic cells into. From chemically to physically induced pluripotency in stem cell. Stimulustriggered fate conversion of somatic cells into pluripotency in chronic wounds in human beings. Stimulustriggered fate conversion of somatic cells. Through realtime imaging of stap cells derived from purified lymphocytes, as well as gene rearrangement analysis, we found that committed somatic cells give rise to stap cells by reprogramming rather than selection. Reprogramming in response to environmental stress has been observed in plants but not so far in mammalian cells. Here we report a unique cellular reprogramming phenomenon, called stimulustriggered acquisition of pluripotency stap, which requires neither nuclear transfer nor the introduction of transcription factors. The fates of the somatic cells that form the bulk of the mammalian body are thought to be largely determined by the time the cellular differentiation processes of development have been completed. It was not clear why ordinary cells do not convert into stem cells when subjected to similar stimuli.
Direct reprogramming of somatic cells into induced neurons. Hereafter, we refer to the fate conversion from somatic cells into pluripotent cells by strong external stimuli such as low ph as stimulustriggered acquisition of pluripotency stap andtheresultantcellsasstapcells. We also demonstrate the derivation of robustly expandable pluripotent cell lines from stap cells. Obokata h1, wakayama t2, sasai y3, kojima k4, vacanti mp5. Article pdf available in journal of krishna institute of medical sciences university 44. Stimulustriggered fate conversion of somatic cells into pluripotency in chronic. Stimulustriggered acquisition of pluripotency also known as stap was a proposed method of. Stimulustriggered fate conversion of somatic cells into pluripotency overview of attention for article published in nature, january 2014 altmetric badge. Pdf here we report a unique cellular reprogramming phenomenon, called stimulustriggered acquisition of pluripotency stap, which requires neither. In stap, strong external stimuli such as a transient lowph stressor reprogrammed mammalian somatic cells, resulting in the generation of pluripotent cells.
Stimulustriggered acquisition of pluripotency wikipedia. Thus, our findings indicate that epigenetic fate determination of. Pdf stimulustriggered fate conversion of somatic cells into. Authors yoshiki sasai pdf 9kb the webpage will open in a new tab. Pdf stimulus triggered fate conversion of somatic cells.
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